By Dorothy Haas

Faculty Mentor: Sarah Smith

Abstract

The column of a High-Performance Liquid Chromatography (HPLC) instrument is the main piece of its separation power. The different elution times of constituents of a sample are based on the components of the column. 3D printing an HPLC column allows for direct manipulation of the column components. The infill pattern can be changed to create more or less interesting pathways, the height of the column can be changed, and the infill percent can be changed to allow larger or smaller molecules through the column. The column can also be printed using different filaments. This research looked at the effects of different infill patterns on a 3D printed column using a carbon-coated filament.